| 結束日期 |
2012-03-31 |
| 計畫單位 |
健康照護學院物理治療學系 |
| 計畫主持人 |
謝悅齡(Yueh-Ling Hsieh) |
| 摘要 |
Background. Nerve inflammation plays an important role in the development and progression of neuropathic pain after chronic constrictive injury (CCI). Recent studies explored hypoxia-inducible factor 1α (HIF-1α) in the process of inflammation. Low-level laser therapy (LLLT) has been suggested to benefit treatment of pain disorders, but few data directly support LLLT for neuropathic pain. Objective. We investigated the effect of LLLT on accumulation of hypoxia-inducible factor-1 alpha (HIF-1α), proinflammatory cytokines tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) for controlling neuropathic pain, as well as on activation of vascular endothelial growth factor (VEGF) and nerve growth factor for promoting functional recovery in rat model of CCI. Methods. CCI was induced by placing four loose ligatures around the sciatic nerve of rats. LLLT (660 nm, 9 J/cm2) at CCI sites was performed after 7 days of CCI. Effects of LLLT in CCI animals were determined by measuring mechanical paw withdrawal threshold (MPWT), sciatic, tibial and peroneal function indexes (SFI, TFI and PFI), and histopathological and immunoassay analyses. Results. Our results demonstrated that LLLT significantly improved MPWT, SFI, TFI and PFI after CCI. LLLT also significantly reduced overexpressions of HIF-1α, TNF-α and IL-1β and increased the amounts of VEGF, NGF and Schwann cells. Conclusions. LLLT can modulate HIF-1α activity and may represent a novel, clinically applicable therapeutic approach for improvement of tissue hypoxia/ischemia and inflammation in nerve entrapment neuropathy as well as for promotion of nerve regeneration, which may lead to sufficient morphologic and functional recovery of the peripheral nerve. |
| 關鍵字 |
Chronic constrictive injury－Low-level laser therapy－Hypoxia-inducible factor 1α－Neuropathic pain－Functional recovery |
| 相關附件 |
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