| 結束日期 |
2012-07-31 |
| 計畫單位 |
健康照護學院醫學檢驗生物技術學系 |
| 計畫主持人 |
姜中人(Chung-Jen Chiang) |
| 摘要 |
Encapsulation of hydrophobic agents with nanocarriers is challenging. To this end, we have sought to nanoscale artificial oil bodies (NOBs) as an alternative vehicle. Caleosin (Cal) is a structural protein associated with plant seed oil bodies. Cal was fused with ZH2 (Cal-ZH2), a bivalent anti-HER2/neu affibody. After overproduction in Escherichia coli, the hybrid protein was isolated to assemble NOBs in one step. Consequently, the zeta potential of resulting NOBs was around -49 mV. Loaded with a hydrophobic fluorescence dye, NOBs assembled by Cal-ZH2 had a size ranging 150-200 nm and were selectively internalized into HER2/neu–positive tumor cells. Moreover, more than 90% cells were invaded by dye-loaded NOBs and the cargo dye was released with time. By the in vitro assay, the release profile of the dye from NOBs exhibited a slow and prolong curve. Overall, these results indicate the potential of Cal-based NOBs as a delivery carrier. |
| 關鍵字 |
artificial oil body, Caleosin, targeted delivery, HER2/neu |
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